room temperature (Proteintech)

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Proteintech room temperature
Room Temperature, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/room+temperature/Collagen+Type+II+Antibody/pmc12963903-85-79-93
Average 96 stars, based on 584 article reviews

room temperature - by Bioz Stars, 2026-09

96/100 stars

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Membrane:

Article Title: LncRNA FAM30A as a potential biomarker associated with periodontitis and its role in inflammatory responses and osteogenesis.
Article Snippet: .. The membrane was blocked with 5% nonfat milk at room temperature (catalog no. 16370-1-AP, Proteintech, USA) for 2 h. The primary antibodies anti-OCN (catalog no. ab93876, Abcam; 1: 1000), anti-OPN (catalog no. ab8488, Abcam; 1:1000), anti-RUNX2 (catalog no. ab76956, Abcam; 1:1000), BMP2 (catalog no. ab284387, Abcam, 1:1000), antiGAPDH (catalog no. ab9485, Abcam; 1:2500, USA). .. The PVDF membrane was incubated with the primary antibody overnight at 4°C, followed by incubation with an HRP-conjugated secondary antibody (catalog no. 7076, Cell Signaling Technology, USA) at room temperature for 1 h. After exposure to Beyo ECL Plus solution (catalog no. P0018S, Beyotime, China), the membrane was visualized using the Syngene Gene Genius gel imaging system.

Incubation:

Article Title: Retrosplenial cortex vulnerability links severe hypoglycemia to cognitive impairment through neuron–microglia crosstalk
Article Snippet: Equal amounts of protein were separated on 8% or 10% sodium dodecyl sulfate‒polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk for one hour at room temperature, followed by overnight incubation at 4°C with primary antibodies [anti-IL-1β (Proteintech, 16806-1-AP, 1:500), anti-TNF-α (Abcam, ab66579, 1:500), anti-IL-6 (Cell Signaling Technology, 12912S, 1:500), anti-Drp1 (Santa Cruz Biotechnology, SC-271583, 1:1,000), anti-phospho-Drp1 (Ser616) (Cell Signaling Technology, 3455S, 1:1,000), anti-PSD95 (Cell Signaling Technology, 3450S, 1:1,000), anti-synaptophysin (Sigma‒Aldrich, S5768, 1:500), anti-Mfn1 (ABclonal, A9880, 1:1,000), anti-Mfn2 (ABclonal, A19678, 1:1,000), anti-OPA1 (ABclonal, A9833, 1:1,000), anti-VDAC1 (Abcam, ab14734, 1:1,000), and anti-β-actin (Santa Cruz Biotechnology, SC-81178, 1:3,000)]. .. After three washes, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [goat anti-mouse (Invitrogen, 31430, 1:2,000) and goat anti-rabbit (Invitrogen, 31460, 1:2,000)] for one hour at room temperature.

Article Title: Ginkgetin alleviates UV-induced skin photoaging by reducing oxidative stress and promoting DNA repair via AKT-mediated homologous recombination repair.
Article Snippet: Background: Ultraviolet (UV)-induced reactive oxygen species (ROS) production, DNA damage, and chronic inflammation lead to skin aging and cancers.. Ginkgetin (GK), a biflavonoid derived from Ginkgo biloba, has known anti-inflammatory and antioxidant activities, but its anti-photoaging effect remains unclear.. Objective: This study aimed to determine whether GK could alleviate UV-induced photoaging by reducing oxidative stress and promoting DNA repair.

Article Title: Manipulation therapy alleviates neuropathic pain via the SOCS1 m 6 A epigenetic loop.
Article Snippet: .. After phosphate buffered saline (PBS) washing, sections were blocked with 10% goat serum for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: rabbit anti-METTL3 (1:200, 15073-1-AP; Proteintech), anti-SOCS1 (1:200, AF5378; Affinity), anti-TLR4 (1:200, 66350-1-Ig; Proteintech); mouse anti-NeuN (1:200, 66836-1-Ig; Proteintech), anti-GFAP (1:200, 60190-1-Ig; PCR: polymerase chain reaction, SOCS1: suppressor of cytokine signaling 1 protein, TLR4: toll-like receptor 4, FTO: fat mass and obesityassociated protein. .. Liping Wu, et al https://doi.org/10.3344/kjp.251246 Proteintech), and anti-IBA1 (1:200, 66827-1-Ig; Proteintech).

Article Title: Integrating Single-Cell and Microarray Data to Explore the Role of Autophagy-Related Gene Atg7 in Osteoporosis
Article Snippet: Equal amounts of protein (30 μg) were separated by SDS-PAGE on a 10% gel and transferred onto PVDF membranes (Millipore). .. Membranes were blocked with 5% skimmed milk (Biofroxx) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies, including anti-β-actin (Proteintech, 20536-1-AP, 1:200,000) and anti-ATG7 (Proteintech, 67341-1-Lg, 1:10,000). .. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech) for 1 hour at room temperature.

Article Title: Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β-TFEB signaling axis.
Article Snippet: The total proteins were extracted from TFEB-EGFP HeLa cells using cell lysis buffer for Western blotting and IP (Beyotime, P0013) with protease and phosphatase inhibitors. .. Protein A/G magnetic beads (HYK0202; MCE) were incubated with GFP antibody [dilution 1:50 (v/v), 50430–2-AP, Proteintech] or rabbit IgG antibody (30000–0-AP; Proteintech) at room temperature for 1 h. Then, protein lysate was added and incubated overnight at 4◦C. ..

Article Title: Development of Intervertebral Disc Organoids through Directed Differentiation of Mesenchymal Stem Cells and Hierarchical 3D Printing
Article Snippet: For IHC, tissue sections were fixed in 4% paraformaldehyde (Biosharp, China) for 24 h at 4 °C, followed by deparaffinization and rehydration. .. Sections were incubated for 1 h at room temperature using COL-1 (Proteintech, 14695-1-AP), COL-2 (Proteintech, 28459–1-AP), and ACAN (Proteintech, 13880-1-AP) as primary antibodies. .. And were then incubated with biotin-labeled goat antirabbit IgG secondary antibody (1:200 dilution; Abcam, ab6720) for 1 h at room temperature.

Saline:

Polymerase Chain Reaction:

Magnetic Beads:

Cell Culture:

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
Article Snippet: Following three gentle PBS washes, intracellular ROS was acquired on a BD LSRFortessa X-20 flow cytometer (excitation 488 nm, emission 525 nm) and quantified in FlowJo to assess oxidative stress across groups. .. Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Eclipse C2 confocal microscope (Japan).

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